a)Use 110 sample number per plate. Table 2 summarizes results of the. 3. high-range. Quant-iT™ PicoGreen ® dsDNA Assay Kit. Rapidly detect as little as 0. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. Table 3. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Unit Size. Rapidly detect as little as 0. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. Rapidly detect as little as 0. The tubes were. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. P11495. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific. 1 mL kit. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. assay. 7 Q33231 6 1,000 41. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. low-range. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. • Specific for dsDNA in the presence of RNA. Optimized for fluorescence microplate readers. Q33130). Using this data as a starting point, the optimal conditions (e. PicoGreen®試薬アッセイ. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 3,201 3. Lambda DNA was used to generate the. Quant-iT PicoGreen dsDNA Assay Kit a. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Prepared DNA concentration of the samples was 250 ng/ mL. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit. 5mL of TE x 5uL PicoReagent. Buy from Supplier. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. 326,933 327K. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. P11495. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. B–F are steps of the assay plate preparation using an test1. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. One microgram of DNA was sheared using an M220. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. 2–100 ng Quant-iT dsDNA HS Assay Kit 0. Quant-iT PicoGreen dsDNA Reagent. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. Catalog Number. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Figure 2. no. • Suitable for use in 96- and 384-well plate formats. 95 mL TE for the . Average 86 stars, based on 1. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. This protocol is a simplified and c. 2 – 100 ng. Q33130). 0 ml 200x in DMSO b. P7589. Facebook. g. 10pg/µl vs. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. PicoGreen dsDNA quantitation reagents are. Figure 2. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. The kit contains. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 04 corresponds to 2 μ g/mL dsDNA solution. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. Table 2 summarizes results of the. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. Mix by gently vortexing for 5 seconds. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 86100 stars. 1. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Rapidly detect as little as 0. 0 fluorometer according to the manufacturer’s protocols; a. 99 to $1999. Q33120). Picogreen assays are compatible with most microplate readers and fluorometers. Serial dilutions were made to give appropriate cell, seeding numbers. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. ZERO BIAS - scores, article reviews, protocol conditions and morePicogreen assay with Qubit platform is considered to be the method of choice for DNA quantification. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. 2 ng to 100 ng of DNA in the assay tube. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The original sequencing data. quant it picogreen dsdna assay kit reagents. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Control samples were not lysed and consisted of free pDNA. 99. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Reagent Search & Data Services Menu Reagent Search. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. 2. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. After a 5-minute. Thermo Fisher quant it picogreen dsdna quantitation assay kit. Tweet. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. 反应次数. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Sample fluorescence was. Bioz Stars score: 99/100, based on 1 PubMed citations. Unit Size. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 5 Q32851 15. The assay is highly selective for double- stranded DNA over RNA, and in the. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. 1 Active esters and kits for labeling proteins and. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Barcoded amplicons were pooled in. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Description. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. 995 mL TE for the . Bioz Stars score: 86/100, based on 1 PubMed citations. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 3. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Rapidly detect as little as 0. Unit Size. High tolerance for contaminants. Quant-iT PicoGreen dsDNA Assay Kit. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. 4). 0 mL to 200 uL to fit a 96-well microplate format. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 500/525. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. Rapidly detect as little as 0. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. • Suitable for use in 96- and 384-well plate formats. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). It is specific for dsDNA in the presence of equimolar amounts of RNA. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. • Specific for dsDNA in the presence of RNA. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. 2. Quant-iT PicoGreen dsDNA Assay Kit. PicoGreen dsDNA quantitation reagents are. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Plasma levels. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. 5), 200X PicoGreen. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. 1 mL kit. Description. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. high-range. in the assay in order to reduce waste and still achieve comparable results. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. All LNP samples were diluted 300-fold in TE buffer and placed on a Corning ® 96 Well Solid Polystyrene Microplate (Sigma-Aldrich, St. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. dsDNA만을 선택적으로 정량. Picogreen assays are compatible with most microplate readers and fluorometers. No. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay. ITSkabir). Figure 2. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. Figure 1. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Mit dem PicoGreen dsDNA. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. 125 to 100 ng/mL. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. DNA standard curve. Determination of DNA Yield. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. 10. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 10. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. Rapidly detect as little as 0. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 作成者 LatB Staff 01. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. 製品概要. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Picogreen assay kit (Invitrogen, P7589) a. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Picogreen assays are compatible with most microplate readers and fluorometers. No. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Rapidly detect as little as 0. P7589. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. Product Type. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. Catalog Number. 99 to $1999. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. No. Katalognummer: P11495. Picogreen assays are compatible with most microplate readers and fluorometers. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Figure 1. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. Bioz Stars score: 86/100, based on 1 PubMed citations. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. PicoGreen assay. Quantitation Range. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Both fluorescent assays were performed with a Qubit fluorometer. Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration. • Suitable for use in 96- and 384-well plate formats. It must be completely liquified before use by allowing it to come to room temperature. 微孔板读数仪. Rapidly detect as little as 0. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Picogreen assays are compatible with most microplate readers and fluorometers. Product Type. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Rapidly detect as little as 0. • Specific for dsDNA in the presence of RNA. 3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 3. • Specific for dsDNA in the presence of RNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2023-11. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. Rapidly detect as little as 0. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 22. 99. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Bioz Stars score: 86/100, based on 1 PubMed citations. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. 99 to $1999. Bioz Stars score: 86/100, based on 1 PubMed citations. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. In addition to the Quant-iT™ dsDNA Broad-Range Assay Kit described here, we also offer the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Cat. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. Prepare only the amount of 1:25 diluted PicoGreen required for that run. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Picogreen assays are compatible with most microplate readers and fluorometers. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. assay. • Suitable for use in 96- and 384-well plate formats. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Quantitation Range. 7. 21 DiB) [22]. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に対応します。. Quantification and normalization of double stranded DNA with minimal set-up time. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. Technical Support Customer Service. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Prepare the 200 fold dilution of pico dye to be kept in the dark. Thermo Fisher quant it picogreen dsdna assay kit.